An H5N1 M2e-based multiple antigenic peptide vaccine confers heterosubtypic protection from lethal infection with pandemic 2009 H1N1 virus.
Identifieur interne : 000202 ( France/Analysis ); précédent : 000201; suivant : 000203An H5N1 M2e-based multiple antigenic peptide vaccine confers heterosubtypic protection from lethal infection with pandemic 2009 H1N1 virus.
Auteurs : Guangyu Zhao [République populaire de Chine] ; Shihui Sun [République populaire de Chine] ; Lanying Du [États-Unis] ; Wenjun Xiao [République populaire de Chine] ; Zhitao Ru [République populaire de Chine] ; Zhihua Kou [République populaire de Chine] ; Yan Guo [République populaire de Chine] ; Hong Yu [République populaire de Chine] ; Shibo Jiang [États-Unis] ; Yuchun Lone [France] ; Bo-Jian Zheng [République populaire de Chine] ; Yusen Zhou [République populaire de Chine]Source :
- Virology Journal [ 1743-422X ] ; 2010.
Abstract
BACKGROUND: A 2009 global influenza pandemic caused by a novel swine-origin H1N1 influenza A virus has posted an increasing threat of a potential pandemic by the highly pathogenic avian influenza (HPAI) H5N1 virus, driving us to develop an influenza vaccine which confers cross-protection against both H5N1 and H1N1 viruses. Previously, we have shown that a tetra-branched multiple antigenic peptide (MAP) vaccine based on the extracellular domain of M2 protein (M2e) from H5N1 virus (H5N1-M2e-MAP) induced strong immune responses and cross-protection against different clades of HPAI H5N1 viruses. In this report, we investigated whether such M2e-MAP presenting the H5N1-M2e consensus sequence can afford heterosubtypic protection from lethal challenge with the pandemic 2009 H1N1 virus. RESULTS: Our results demonstrated that H5N1-M2e-MAP plus Freund's or aluminum adjuvant induced strong cross-reactive IgG antibody responses against M2e of the pandemic H1N1 virus which contains one amino acid variation with M2e of H5N1 at position 13. These cross-reactive antibodies may maintain for 6 months and bounced back quickly to the previous high level after the 2nd boost administered 2 weeks before virus challenge. H5N1-M2e-MAP could afford heterosubtypic protection against lethal challenge with pandemic H1N1 virus, showing significant decrease of viral replications and obvious alleviation of histopathological damages in the challenged mouse lungs. 100% and 80% of the H5N1-M2e-MAP-vaccinated mice with Freund's and aluminum adjuvant, respectively, survived the lethal challenge with pandemic H1N1 virus. CONCLUSIONS: Our results suggest that H5N1-M2e-MAP has a great potential to prevent the threat from re-emergence of pandemic H1N1 influenza and possible novel influenza pandemic due to the reassortment of HPAI H5N1 virus with the 2009 swine-origin H1N1 influenza virus.
Url:
DOI: 10.1186/1743-422X-7-151
Affiliations:
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Hal:inserm-00668214Le document en format XML
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<author><name sortKey="Yu, Hong" sort="Yu, Hong" uniqKey="Yu H" first="Hong" last="Yu">Hong Yu</name>
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<desc> <address> <addrLine>Beijing 100071</addrLine>
<country key="CN"></country>
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<desc> <address> <country key="CN"></country>
</address>
</desc>
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</tutelles>
</hal:affiliation>
<country>République populaire de Chine</country>
</affiliation>
</author>
<author><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-183383" status="INCOMING"> <orgName>Lindsley F. Kimball Research Institute</orgName>
<desc> <address> <addrLine>New York, NY 10065</addrLine>
<country key="US"></country>
</address>
</desc>
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<tutelles><tutelle active="#struct-345810" type="direct"><org type="institution" xml:id="struct-345810" status="VALID"> <orgName>New York Blood Center</orgName>
<desc> <address> <addrLine>310 E 67th StNew York, NY 10065</addrLine>
<country key="US"></country>
</address>
<ref type="url">https://nybloodcenter.org/</ref>
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</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>États-Unis</country>
</affiliation>
</author>
<author><name sortKey="Lone, Yuchun" sort="Lone, Yuchun" uniqKey="Lone Y" first="Yuchun" last="Lone">Yuchun Lone</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-223704" status="VALID"> <orgName>Régulation de la survie cellulaire et des allogreffes</orgName>
<desc> <address> <addrLine>Batiment Inserm Lavoisier - 14, avenue Paul Vaillant-Couturier 94800 Villejuif France</addrLine>
<country key="FR"></country>
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<tutelles><tutelle active="#struct-92966" type="direct"><org type="institution" xml:id="struct-92966" status="VALID"> <orgName>Université Paris-Sud - Paris 11</orgName>
<orgName type="acronym">UP11</orgName>
<desc> <address> <addrLine>Bâtiment 300 - 91405 Orsay cedex</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.u-psud.fr/</ref>
</desc>
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</tutelle>
<tutelle active="#struct-300068" type="direct"><org type="regroupinstitution" xml:id="struct-300068" status="VALID"> <orgName>Assistance publique - Hôpitaux de Paris (AP-HP)</orgName>
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<desc> <address> <country key="FR"></country>
</address>
</desc>
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<tutelle active="#struct-300153" type="direct"><org type="institution" xml:id="struct-300153" status="VALID"> <orgName>Hôpital Paul Brousse</orgName>
<desc> <address> <addrLine>12 Avenue Paul Vaillant Couturier, 94800 Villejuif</addrLine>
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</desc>
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<tutelle name="U1014" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno>
<orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
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<desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine>
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<ref type="url">http://www.inserm.fr</ref>
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</org>
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</hal:affiliation>
<country>France</country>
</affiliation>
</author>
<author><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-183385" status="INCOMING"> <orgName>Department of Microbiology</orgName>
<desc> <address> <addrLine>Hong Kong</addrLine>
<country key="CN"></country>
</address>
</desc>
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<orgName type="acronym">HKU</orgName>
<desc> <address> <addrLine>Pokfulam, Hong Kong</addrLine>
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</address>
<ref type="url">http://www.hku.hk/</ref>
</desc>
</org>
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</hal:affiliation>
<country>République populaire de Chine</country>
</affiliation>
</author>
<author><name sortKey="Zhou, Yusen" sort="Zhou, Yusen" uniqKey="Zhou Y" first="Yusen" last="Zhou">Yusen Zhou</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-183382" status="INCOMING"> <orgName>State Key Laboratory of Pathogen and Biosecurity</orgName>
<desc> <address> <addrLine>Beijing 100071</addrLine>
<country key="CN"></country>
</address>
</desc>
<listRelation> <relation active="#struct-306858" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-306858" type="direct"><org type="institution" xml:id="struct-306858" status="INCOMING"> <orgName>Beijing Institute of Microbiology and Epidemiology</orgName>
<desc> <address> <country key="CN"></country>
</address>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>République populaire de Chine</country>
</affiliation>
</author>
</analytic>
<idno type="DOI">10.1186/1743-422X-7-151</idno>
<series><title level="j">Virology Journal</title>
<idno type="ISSN">1743-422X</idno>
<imprint><date type="datePub">2010</date>
</imprint>
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<front><div type="abstract" xml:lang="en"> <p>BACKGROUND: A 2009 global influenza pandemic caused by a novel swine-origin H1N1 influenza A virus has posted an increasing threat of a potential pandemic by the highly pathogenic avian influenza (HPAI) H5N1 virus, driving us to develop an influenza vaccine which confers cross-protection against both H5N1 and H1N1 viruses. Previously, we have shown that a tetra-branched multiple antigenic peptide (MAP) vaccine based on the extracellular domain of M2 protein (M2e) from H5N1 virus (H5N1-M2e-MAP) induced strong immune responses and cross-protection against different clades of HPAI H5N1 viruses. In this report, we investigated whether such M2e-MAP presenting the H5N1-M2e consensus sequence can afford heterosubtypic protection from lethal challenge with the pandemic 2009 H1N1 virus. RESULTS: Our results demonstrated that H5N1-M2e-MAP plus Freund's or aluminum adjuvant induced strong cross-reactive IgG antibody responses against M2e of the pandemic H1N1 virus which contains one amino acid variation with M2e of H5N1 at position 13. These cross-reactive antibodies may maintain for 6 months and bounced back quickly to the previous high level after the 2nd boost administered 2 weeks before virus challenge. H5N1-M2e-MAP could afford heterosubtypic protection against lethal challenge with pandemic H1N1 virus, showing significant decrease of viral replications and obvious alleviation of histopathological damages in the challenged mouse lungs. 100% and 80% of the H5N1-M2e-MAP-vaccinated mice with Freund's and aluminum adjuvant, respectively, survived the lethal challenge with pandemic H1N1 virus. CONCLUSIONS: Our results suggest that H5N1-M2e-MAP has a great potential to prevent the threat from re-emergence of pandemic H1N1 influenza and possible novel influenza pandemic due to the reassortment of HPAI H5N1 virus with the 2009 swine-origin H1N1 influenza virus.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>France</li>
<li>République populaire de Chine</li>
<li>États-Unis</li>
</country>
</list>
<tree><country name="République populaire de Chine"><noRegion><name sortKey="Zhao, Guangyu" sort="Zhao, Guangyu" uniqKey="Zhao G" first="Guangyu" last="Zhao">Guangyu Zhao</name>
</noRegion>
<name sortKey="Guo, Yan" sort="Guo, Yan" uniqKey="Guo Y" first="Yan" last="Guo">Yan Guo</name>
<name sortKey="Kou, Zhihua" sort="Kou, Zhihua" uniqKey="Kou Z" first="Zhihua" last="Kou">Zhihua Kou</name>
<name sortKey="Ru, Zhitao" sort="Ru, Zhitao" uniqKey="Ru Z" first="Zhitao" last="Ru">Zhitao Ru</name>
<name sortKey="Sun, Shihui" sort="Sun, Shihui" uniqKey="Sun S" first="Shihui" last="Sun">Shihui Sun</name>
<name sortKey="Xiao, Wenjun" sort="Xiao, Wenjun" uniqKey="Xiao W" first="Wenjun" last="Xiao">Wenjun Xiao</name>
<name sortKey="Yu, Hong" sort="Yu, Hong" uniqKey="Yu H" first="Hong" last="Yu">Hong Yu</name>
<name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name>
<name sortKey="Zhou, Yusen" sort="Zhou, Yusen" uniqKey="Zhou Y" first="Yusen" last="Zhou">Yusen Zhou</name>
</country>
<country name="États-Unis"><noRegion><name sortKey="Du, Lanying" sort="Du, Lanying" uniqKey="Du L" first="Lanying" last="Du">Lanying Du</name>
</noRegion>
<name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name>
</country>
<country name="France"><noRegion><name sortKey="Lone, Yuchun" sort="Lone, Yuchun" uniqKey="Lone Y" first="Yuchun" last="Lone">Yuchun Lone</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
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